69587
Sigma
2′-(4-Methylumbelliferyl)-α-D -N-acetylneuraminic acid sodium salt hydrate
BioChemika, for fluorescence, ≥96.5% (HPLC)
| Synonym: | 4-Methylumbelliferyl-N-acetyl-α- |
| CAS Number: | 76204-02-9 (anhydrous) |
| Linear Formula: | C21H24NNaO11 · xH2O |
| Molecular Weight: | 489.41 (anhydrous basis) |
Description
| Biochem/physiol Actions | Substrate for fluorometric assay of neuraminidase.1 Used for fluorescent staining of sialidases in PAGE.2 |
Properties
| grade | for fluorescence |
| product line | BioChemika |
| assay | ≥96.5% (HPLC) |
| total impurities | ≤0.5% free 4-methylumbelliferone |
| ≤10% water | |
| fluorescence | λex 365 nm; λem 445 nm (after cleavage by neuraminidase) |
| solubility | H2O: 50 mg/mL, clear, very slightly yellow |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
References
| Cited Reference | 1. Potier, M., et al. Anal. Biochem. 94, 287, (1979) |
| 2. W. Berg, Fluorescent staining of sialidases in PAGE. Anal. Biochem. 145, 339, (1985) | |
| reference | Myers, R.W., et al. Anal. Biochem. 101, 166, (1980) |
